DTT

Dithiothreitol (DTT)

Active Product:

Appearance: powder white

Brand name(s):
Chemical Name: (2S,3S)-1,4-Bis-sulfanylbutane-2,3-diol

Molecular Weight: M.W. 154.253
Solubility: H₂O: 0.1 M at 20 °C, Aliquot and store at -20 °C
Storage: - 20 °C

Target: Disulfide bonds

Comment	Structure
DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins.DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).		Go to the site Advertise here
		Go to the site Advertise here
	Synonym: Threo-1,4-dimercaptobutane-2,3-diol DL-Threo-1,4-dimercapto-2,3-butanediol OR ,4-Dithio-DL-threitol ±-Threo-1,4-dimercapto-2,3-butanediol OR Cleland's reagent OR Reductacryl Molecular Formula: C₄H₁₀O₂S₂
Reduction of a typical disulfide bond by DTT via two sequential thiol-disulfide exchange reactions

References
- Cleland, W.W. (April 1964). "Dithiothreitol, A New Protective Reagent for SH Groups". Biochemistry 3: 480–2. - Ruegg UT and Rudinger J. (1977) "Cleavage of disulfide bonds in proteins", Methods Enzymol., 47, 111.